Alpco 80-CPTHU-E01.1 C-Peptide ELISA | 人C肽酶免試劑盒，96 wells

Alpco公司從成立初期就致力于生物醫藥領域各種免疫檢測試劑盒的研發與生產，力求為廣大客戶提供高質量的免疫檢測產品。
Alpco產品種類多樣，可測樣品來源包括人、大/小鼠、豚鼠、兔、豬、羊、狗、猴等，涉及神經生物學、骨代謝、心血管疾病和氧化應激、細胞因子與信號轉導、糖尿病和肥胖癥、內分泌學、甲狀腺功能、腸胃病學、免疫學、生殖激素、抗體和抗原、分子診斷等多個研究領域，尤以糖尿病和肥胖癥研究領域見長。

我們北京諾博萊德科技有限公司作為Alpco公司的經銷商為客戶提供，正規進口，貨期穩定，折扣好的服務標準。

Alpco 80-CPTHU-E01.1 C-Peptide ELISA，人C肽酶免試劑盒，96 well，產品簡介：

產品品牌：Alpco

產品名稱：人C肽酶免試劑盒

英文名稱：Alpco 80-CPTHU-E01.1 C-Peptide ELISA

產品貨號：80-CPTHU-E01.1

產品規格：96 well

Alpco 80-CPTHU-E01.1 C-Peptide ELISA，人C肽酶免試劑盒，96 well，產品說明檢測原理：

ALPCO C-肽ELISA是一個夾層式免疫檢測試劑盒。96孔微孔板涂有一層C-肽特異性單克隆抗體。將標準物，對照品和樣品一并添加到微型板小孔中與檢測緩沖液混合。然后將微孔板放在搖床上室溫溫育1小時，700－900轉/分鐘。*次溫育結束，用清洗緩沖液清洗小孔并烘干。添加結合物（配制方法參照說明書），微孔板放在搖床上第二次室溫溫育1小時，700－900轉/分鐘。添加TMB作用底物，放在微孔板搖床上室溫溫育15分鐘，700－900轉/分鐘。第三次溫育結束，添加反應終止液，調節分光光度計到450nm測定OD值。由于發光強度與樣品中C-肽含量成比例，因此可以根據OD值確定C-肽含量。

§ 檢測過程：各添加25μl標準物，對照物和樣品→加50µl檢測緩沖液→微孔板搖床室溫溫育1小時，700－900rpm→清洗緩沖液清洗6次→加100µl結合物（配制方法參照說明書）→微孔板搖床室溫溫育1小時，700－900rpm→清洗緩沖液清洗6次→添加100µl TMB作用底物→微孔板搖床室溫溫育15分鐘，700－900rpm→添加100µl反應終止液→分光光度計450nm讀數

ALPCO C-peptide ELISA kit英文簡述：

The ALPCO C-peptide ELISA kit is a FDA registered in vitro diagnostic tool for the quantification of human C-peptide in a clinical setting or research laboratory. Utilizing a dual-monoclonal antibody sandwich ELISA format, bi-level control set, and 96-well microplate comprising removable strips, a single C-peptide ELISA kit has the performance characteristics and flexibility necessary to confidently measure up to 40 samples in duplicate. The shelf-life of the components of the C-Peptide ELISA and the resealable microplate pouch allow for convenient storage for future use if the entire kit is not needed at one time.

INTENDED USE：The ALPCO C-peptide ELISA is designed for the quantitative determination of C-peptide in human serum and plasma.

PRINCIPLE OF THE ASSAY：The ALPCO C-peptide ELISA is a sandwich type immunoassay. The 96-well microplate is coated with a monoclonal antibody specific for C-peptide. The standards, controls, and samples are added to the microplate wells with the Assay Buffer. The microplate is then incubated at room temperature on a microplate shaker at 700-900 rpm. After the first incubation is complete, the wells are washed with Wash Buffer and blotted dry. The Conjugate is then added, and the microplate is incubated a second time on a microplate shaker at 700-900 rpm, washed, and blotted dry. TMB Substrate is added, and the microplate is incubated a third time at room temperature on a microplate shaker at 700-900 rpm. Once the third incubation is complete, Stop Solution is added, and the optical density （OD） is measured by a spectrophotometer at 450 nm. The intensity of the color generated is directly proportional to the amount of C-peptide in the sample.

Alpco品牌其它部分產品貨號：

如需其它產品請聯系本店客服。