GK034 ScienCell 人肺纖維化qPCR檢測試劑盒,Human Pulmonary Fibrosis qPCR Array Kit

ScienCell 研究實(shí)驗(yàn)室（ScienCell）是一家生物技術(shù)公司，其使命是實(shí)驗(yàn)和治療用細(xì)胞和細(xì)胞相關(guān)產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質(zhì)量的正常的人類和動物細(xì)胞，細(xì)胞培養(yǎng)基和試劑，培養(yǎng)基添加因子，細(xì)胞來源的RNA，cDNA和蛋白質(zhì)。ScienCell的科學(xué)家們以改善生活質(zhì)量，實(shí)現(xiàn)細(xì)胞的能力，打擊人類退化性疾病為目的研究和開發(fā)各種細(xì)胞治療策略。ScienCell總部設(shè)在美國加利福尼亞州圣地亞哥，公司成立于1999年。

北京諾博萊德作為ScienCell品牌的經(jīng)銷商，為客戶提供，正規(guī)進(jìn)口，貨期穩(wěn)定，折扣好的服務(wù)標(biāo)準(zhǔn)。

GK034 ScienCell 人肺纖維化qPCR檢測試劑盒，Human Pulmonary Fibrosis qPCR Array Kit，產(chǎn)品簡介：

產(chǎn)品品牌: ScienCell

產(chǎn)品貨號：GK034

產(chǎn)品名稱：GK034 ScienCell 人肺纖維化qPCR檢測試劑盒，Human Pulmonary Fibrosis qPCR Array Kit

產(chǎn)品規(guī)格：One 96-well plate, one 20ul reaction per well

GK034 ScienCell 人肺纖維化qPCR檢測試劑盒，Human Pulmonary Fibrosis qPCR Array Kit， 產(chǎn)品說明：

ScienCell的GeneQuery™人肺纖維化qPCR陣列試劑盒（GQH-PFS）旨在促進(jìn)88個參與肺纖維化的關(guān)鍵基因的基因表達(dá)譜分析。肺纖維化是由傷口愈合和組織重塑過程中細(xì)胞外基質(zhì)（ECM）沉積過多引起的。隨著時間的推移，肺部瘢痕形成會導(dǎo)致組織增厚、僵硬和呼吸問題。如何根據(jù)其功能對所包含的基因進(jìn)行分組的簡要示例如下所示：  

肺表面活性蛋白：SFTPA2，SFTPAC

炎癥趨化因子：CCL2，CCL3，CCL11，CCR2，CCR3，CXCL8

炎性細(xì)胞因子：PDGFA，CSF2，IL5，IL6，IL13

ECM 重塑： LOX， MMP1， MMP2， MMP8， PLAT， PLAU， PLG， SERPINA1， SERPINE2， TIMP1， TIMP2， TIMP3

生長因子：EGF，IGF1，HGF，FGF1，FGF2，AGT，EDN1，CTGF

TGFB信號通路：SKIL，SPP1，TGFB1，TGFB2

GeneQuery™ qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒，每個孔包含一個引物組，可以特異性識別并有效擴(kuò)增靶基因的 cDNA。精心設(shè)計的引物確保：（i）qPCR分析中的優(yōu)良退火溫度為65°C（含2 mM Mg2+，不含DMSO）;（ii）除非另有說明，否則引物組可識別靶基因的所有已知轉(zhuǎn)錄本變體;（iii）只有一個基因被擴(kuò)增。每個引物組都已通過qPCR和熔解曲線分析和凝膠電泳進(jìn)行了驗(yàn)證。

ScienCell's GeneQuery™ Human Pulmonary Fibrosis qPCR Array Kit (GQH-PFS) is designed to facilitate gene expression profiling of 88 key genes involved in pulmonary fibrosis. Pulmonary fibrosis is caused by excessive extracellular matrix (ECM) deposition during wound healing and tissue remodeling. The scarring of lung leads to thickened, stiff tissues and respiratory problems over time. Brief examples of how included genes may be grouped according to their functions are shown below:    

·         Pulmonary surfactant proteins: SFTPA2, SFTPAC

·         Inflammatory chemokines: CCL2, CCL3, CCL11, CCR2, CCR3, CXCL8

·         Inflammatory cytokines: PDGFA, CSF2, IL5, IL6, IL13

·         ECM remodeling: LOX, MMP1, MMP2, MMP8, PLAT, PLAU, PLG, SERPINA1, SERPINE2, TIMP1, TIMP2, TIMP3

·         Growth factors: EGF, IGF1, HGF, FGF1, FGF2, AGT, EDN1, CTGF

·         TGFB signaling pathway: SKIL, SPP1, TGFB1, TGFB2

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

基因查詢™人肺纖維化qPCR陣列板布局*（8 個粗體和斜體控件）

提示：基因選擇可能會根據(jù)新的研究和開發(fā)進(jìn)行更新

ScienCell基因表達(dá)分析產(chǎn)品訂購信息：

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